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<div id="right">...Cellular Technologies
Cell line development
Engineered cell-based assays
Cell based assays are an essential tool in understanding biological pathways especially when linked to high throughput readouts. Native cell lines or cells engineered to express foreign reporter genes can be used to read out a biological target or pathway or to confirm the activity and mechanism of action of drug candidates. Dragonfly has extensive experience in establishing known cell-based assays or developing new ones.
Examples of engineered cells and assays which Dragonfly has experience in include:
Heterologous or chimeric receptor growth factor assays. Growth factor dependent cells can convenient cellular bioassays for growth factors or cytokines. Dragonfly has engineered heterologous human or rodent receptors into factor dependent cells as stable transformants to provide a readouts for ligand induced receptor activation or to assay small molecule or antibody antagonists. We have also engineered chimeric receptors with novel extracellular domains linked to a signaling intracellular domain to derive ligand dependent cell lines.
Engineered reporter cell assays. Reporter genes may be used to monitor promoter activity, the expression of tagged proteins, or to read out a biological pathway. Dragonfly has experience with multiple cell types engineered to express a variety of epitope tagged proteins or reporter proteins adapted to high throughput, 96 well assays. Examples of epitope tags or reporters we have worked with include: FLAG, His6, myc, GFP and lac Z.
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