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1. Phytochem Anal. 2011 Jan 24. doi: 10.1002/pca.1274. [Epub ahead of print]
Isolation of four high-purity dammarane saponins from extract of Panax
notoginseng by centrifugal partition chromatography coupled with evaporative
light scattering detection in one operation.
Wang J, Liu CM, Li L, Bai HL.
Department of Chemistry, Changchun Normal University, Changchun 130032, China.
Introduction - Centrifugal partition chromatography (CPC), as a continuous
liquid-liquid partition chromatography with no solid support matrix, combined
with evaporative light scattering detection (ELSD) was employed for systematic
separation and purification of weak-chromophoric saponins from a highly valued
and important traditional Chinese herbal medicine, Panax notoginseng. Objective -
To separate and isolate high-purity saponins from extract of Panax notoginseng
using CPC-ELSD with a simple and low toxicity solvent system. Methodology -
Samples were preparaed by extracting the root material with acetone, treated with
n-butanol and then freeze-dried. CPC-ELSD was applied in the separation and
detection of notoginsenoside and ginsenosides from extract of Panax notoginseng
using a solvent system composed of ethyl acetate-n-butanol-water (1:1:2, v/v/v).
The saponins were analysed and identified by their retention time with
high-performance liquid chromatography (HPLC) coupled with ELSD, as well as
electrospray ionisation tandem mass spectrometry (ESI-MS(n) ) in the negative and
positive ion modes with the authentic standards. Results - A total of 9.6 mg of
notoginsenoside R(1) , 67.8 mg of ginsenoside Rg(1) , 2.3 mg of Re and 286.5 mg
of Rb(1) were purified from 487.2 mg of n-butanol extract of P. notoginseng. The
purities of obtained saponins in a single run were assessed to be over 98% by
HPLC-ELSD. Conclusion - CPC-ELSD was proved to be a very fast and efficient tool
for separation of high-purity dammarane saponins. Copyright © 2011 John Wiley &
PMID: 21259375 [PubMed - as supplied by publisher]
2. Chin Med. 2011 Jan 24;6(1):5.
Saponin accumulation in the seedling root of Panax notoginseng.
Wang D, Zhu H, Chen K, Xu M, Zhang Y, Yang C.
State Key Laboratory of Phytochemistry and Plant Resources of West China, Kunming
Institute of Botany, Chinese Academy of Sciences, Kunming 650204, PR China.
ABSTRACT:BACKGROUND: Panax notoginseng is an important Chinese medicinal plant.
Dammarene-type triterpenoid saponins are main pharmacologically effective
compounds in P. notoginseng. This study aims to investigate the formation and
accumulation of saponins in P. notoginseng roots during germination and juvenile
METHODS: P. notoginseng seeds were collected and stored in wet sand. After
germination, the seedlings were transplanted into a soil nursery bed and
cultivated for one year. During this period, samples were collected every month
and the concentrations of ginsengnosides Rg1, Re, Rb1, Rd and notoginsengnoside
R1 were determined by HPLC.
RESULTS: There was little saponin in the P. notoginseng seed. The chemical
composition of seed was different from that of root. After germination, Rb1, Rg1,
Re, Rd and R1 appeared successively in the seedling root. And in the
five-month-old root, all these five main saponins came into existence. The
accumulation of saponins in P. notoginseng root was affected by seasons.
CONCLUSION: The accumulation of saponins showed a time-dependent increase after
germination of P. notoginseng.
PMID: 21255468 [PubMed - in process]